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The 100 most highly cited papers of all time

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To mark the anniversary, Nature magazine asked Thomson Reuters to list the 100 most highly cited papers of all time. The search covered all of Thomson Reuter’s Web of Science, an online version of the SCI that also includes databases covering the social sciences, arts and humanities, conference proceedings and some books. It lists papers published from 1900 to the present day.

 
Title of the paperRankSujet
Protein measurement with the folin phenol reagent.1Biology lab techniqueVoir plus
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.2Biology lab techniqueVoir plus
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.3Biology lab techniqueVoir plus
DNA sequencing with chain-terminating inhibitors.4Biology lab techniqueVoir plus
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.5Biology lab techniqueVoir plus
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.6Biology lab techniqueVoir plus
Development of the Colle-Salvetti correlation-energy formula into a functional of the electron density.7Physical chemistryVoir plus
Density-functional thermochemistry. III. The role of exact exchange.8Physical chemistryVoir plus
A simple method for the isolation and purification of total lipides from animal tissues.9Biology lab techniqueVoir plus
Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting - position-specific gap penalties and weight matrix choice.10BioinformaticsVoir plus
Nonparametric estimation from incomplete observations.11Medical statisticsVoir plus
Basic local alignment search tool.12BioinformaticsVoir plus
A short history of SHELX.13CrystallographyVoir plus
Gapped BLAST and PSI-BLAST: A new generation of protein database search programs.14BioinformaticsVoir plus
A revised medium for rapid growth and bio assays with tobacco tissue cultures.15Biology lab techniqueVoir plus
Generalized gradient approximation made simple.16Physical chemistryVoir plus
Mini-mental state: A practical method for grading cognitive state of patients for clinician.17Psychology/psychiatryVoir plus
A rapid method of total lipid extraction and purification.18Biology lab techniqueVoir plus
Detection of specific sequences among DNA fragments separated by gel-electrophoresis.19Biology lab techniqueVoir plus
The neighbor-joining method: A new method for reconstructing phylogenetic trees.20PhylogeneticsVoir plus
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